Supporters will face enhanced security checks, an alcohol ban and a “robust policing plan” when West Ham host Chelsea in the EFL Cup on Wednesday.
Chelsea have been allocated more than 5,000 tickets for the fourth-round tie, deemed high risk because of a history of animosity between fans.
Since West Ham relocated to London Stadium this season, 23 banning orders have been issued.
Nine arrests have been made outside the ground since the move from Upton Park.
For the visit of Chelsea, fans should expect an “enhanced body search policy” at the turnstile, while no alcohol will be sold before the match from mobile bars outside the stadium.
A spokesperson for partners in the stadium – West Ham, E20 Stadium LLP and London Stadium 185 – said “significant resources” had been applied to the fixture with a “robust policing plan in place”.
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Until now, the Metropolitan Police have not deployed officers inside the stadium because the radio system emergency services use to communicate will not be operational until 2017.
But the statement added a “tactical solution” was now in place for the “safe deployment of officers in the stadium”.
Three people were arrested after West Ham’s Premier League draw with Middlesbrough this month, while there were clashes in the stands during the defeat by Watford in September.
Recent matches have featured increased segregation of opposing fans and more stewards.